Abstract: With the gradual depletion of easily separated and processed gold ore resources in a gold deposit in Shandong Province, and the continuous decline in head grade, a blending strategy involving gold resources from different ore veins was adopted. However, product indices exhibited large fluctuations. To determine the cause, a process mineralogy study was conducted, along with grindability and flotation tests. The test results showed that the blended ore had a gold grade of 3.45 g/t. Gold minerals mainly occurred in close association with pyrite, predominantly as inclusions, with a small proportion as intergrowths. Bond ball mill work index tests indicated that the ore sample had a work index (W_ib) of 23.36 kW·h/t, significantly higher than that of the original single-vein ore sample. Under the conditions of 180 g/t of xanthate, 2 g/t of dithiophosphate, 30 g/t of No. 2 oil, and a grinding fineness of −0.074 mm accounting for 60.32 %, a one rougher-two cleaner-three scavenger flowsheet produced a concentrate with a gold grade of 53.23 g/t, and the recovery rate increased from 90.08 % to 94.11 %. In addition, tests on the dithiophosphate dosage showed that the amount should not be excessive; an excessive dosage led to abundant froth, increasing the difficulty of the flotation operation.